Table 4.
Cell growth, glucose consumption, ethanol formation, and furan aldehydes conversion of co-expression recombinant strains
| Inhibitors | Recombinant strains | Cell growth (OD600) | Glucose consumption (g/L) | Ethanol production (g/L) | Inhibitor conversion (g/L) |
|---|---|---|---|---|---|
| Furfural | ZM4(pHW20a-ZMO1771) | 0.259 ± 0.002 | 3.84 ± 0.11 | 1.14 ± 0.03 | 1.31 ± 0.25 |
| ZM4(pHW20a-ZMO1771-pntAB) | 0.252 ± 0.011 | 3.44 ± 0.09 | 1.04 ± 0.02 | 1.49 ± 0.03 | |
| ZM4(pHW20a-ZMO1771-udhA) | 0.243 ± 0.006 | 4.02 ± 0.59 | 0.91 ± 0.05 | 1.57 ± 0.27 | |
| ZM4(pHW20a-ZMO1771-ZMO0367) | 0.230 ± 0.005 | 3.52 ± 0.34 | 1.14 ± 0.07 | 1.42 ± 0.11 | |
| HMF | ZM4(pHW20a-ZMO1771) | 0.357 ± 0.004 | 6.43 ± 0.76 | 1.99 ± 0.08 | 1.97 ± 0.18 |
| ZM4(pHW20a-ZMO1771-pntAB) | 0.421 ± 0.025 | 6.50 ± 0.13 | 2.28 ± 0.15 | 2.23 ± 0.14 | |
| ZM4(pHW20a-ZMO1771-udhA) | 0.470 ± 0.036 | 7.84 ± 0.63 | 2.70 ± 0.08 | 2.72 ± 0.05 | |
| ZM4(pHW20a-ZMO1771-ZMO0367) | 0.391 ± 0.009 | 5.66 ± 0.25 | 2.18 ± 0.16 | 2.02 ± 0.01 |
In RM medium containing 2 g/L of furfural or 4 g/L of HMF at 30 °C. The fermentation performance was detected at the mid-log phase, where 12 h for furfural and 16 h for HMF. Standard deviations were derived from at least two independent determinations