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. 2017 Jan 31;16:16. doi: 10.1186/s12934-017-0634-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Chi18H8 production at 3 L bench-bioreactor scale. a Chi18H8 production (mg/g cell) into insoluble fractions was determined by densitometric analysis of proteins separated through SDS-PAGE. Chitinolytic activity was measured by fluorimetric assay on 4-MU-(GlcNAc)₂. b Time course of pH, pO₂, temperature and wet weight. Induction of protein expression with 0.4 mM IPTG is indicated by an arrow. The values represent the mean of three experiments. Standard error never exceeded 10%