Table 1.
Chi18H8 solubilization from IBs of E. coli BL21 Star™(DE3)/pET24b(+)::chi18H8 cells in acidic pH conditions
| Solubilization agent | Concentration (mM) | Solubilization yield (%) | Specific activity (U/mgChi18H8)a |
|---|---|---|---|
| HCl | 10 | 93 ± 4.0 | 29.6 ± 1.2 |
| 100 | 20 ± 2.0 | 15.9 ± 0.9 | |
| Lactic acid | 10 | 82 ± 3.5 | 40.7 ± 1.4 |
| 100 | 60 ± 6.5 | 30.7 ± 1.3 |
Solubilization yield was estimated by densitometric analysis of proteins separated by SDS-PAGE. Enzyme activity was measured by fluorimetric assay on 4-MU-(GlcNAc)2
aValues represent the mean of three independent experiments (mean ± standard error)