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Details of the primers and restriction enzymes used in CAPS marker development
| Gene | Primer name | Primer sequence | Restriction enzyme | Amplicon size (bp) |
|---|---|---|---|---|
| FAE1.1 | CAPS591 | F-TCGTGGCTTGACTTCTTGAG R-GGACCTATTATCACCAGCGTAAA |
Hpy99 I | 432 |
| CAPS1265 | F-ACGTTAGGTCCGTTGATTCTTC R-GGGTATCTGTCGATGCAATGT |
BG1II | 427 | |
| FAE1.2 | CAPS237 | F-TAACCATCGCTCCACTCTTTG R-TCAAGAAGTCAAGCCACGAC |
MnlI | 219 |
