Table 1.

Classification and general features of S. thermophilus strain ACA-DC 2 according to the MIGS recommendations [39]

MIGS ID	Property	Term	Evidence codea
	Classification	Domain Bacteria	TAS [40]
		Phylum Firmicutes	TAS [41, 42]
		Class Bacilli	TAS [43, 44]
		Order Lactobacillales	TAS [44, 45]
		Family Streptococcacceae	TAS [46–48]
		Genus Streptococcus	TAS [47, 49, 50]
		Species Streptococcus thermophilus	TAS [47, 51, 52]
		Strain: ACA-DC 2	TAS (this study)
	Gram stain	Positive	IDA
	Cell shape	Coccus	IDA
	Motility	Non-motile	IDA
	Sporulation	Non-sporulating	NAS
	Temperature range	30–50 °C	TAS [51]
	Optimum temperature	42 °C	TAS [53]
	pH range; Optimum	5–7; 6.5	TAS [53]
	Carbon source	lactose; saccharose; d-glucose; galactose	IDA
MIGS-6	Habitat	Yogurt	TAS [13, 14]
MIGS-6.3	Salinity	2% NaCl (w/v)	TAS [51]
MIGS-22	Oxygen requirement	Microaerophilic	TAS [51]
MIGS-15	Biotic relationship	Free-living	NAS
MIGS-14	Pathogenicity	Non-pathogen	NAS
MIGS-4	Geographic location	Greece	TAS [13, 14]
MIGS-5	Sample collection	1988	NAS
MIGS-4.1	Latitude	Unknown
MIGS-4.2	Longitude	Unknown
MIGS-4.4	Altitude	Unknown

^aEvidence codes - IDA inferred from direct assay, TAS traceable author statement (i.e., a direct report exists in the literature), NAS non-traceable author statement (i.e., not directly observed for the living, isolated sample, but based on a generally accepted property for the species, or anecdotal evidence). These evidence codes are from the Gene Ontology project [54]