Table 5.
Validation of the DEGs with qRT-PCR experiment
| Unigene ID | Gene name | Fold change by RNA-Seq | Fold change by qRT-PCR |
|---|---|---|---|
| d2.comp51231_c0_seq1 | GST | 8.79 | 10.85 ± 0.941 |
| d2.comp347335_c0_seq1 | GPX | 6.23 | 5.23 ± 0.115 |
| CL3838contig1 | ADH1 | 2.29 | 1.74 ± 0.086 |
| d2.comp60738_c0_seq1 | LDH | 6.07 | 3.47 ± 0.343 |
| d2.comp51785_c0_seq1 | AlaAT | 7.91 | 9.82 ± 0.124 |
| d2.comp63174_c0_seq1 | AspAT | 2.10 | 1.46 ± 0.047 |
| d2.comp58507_c0_seq3 | PsaL | 7.94 | 11.60 ± 0.029 |
| d2.comp47781_c0_seq1 | PetF | 6.23 | 3.92 ± 0.279 |
Note: The confirmation of expression level of candidate genes examined by real-time PCRs with three technical replications. DEG differentially expressed genes