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. 2016 Jun 28;35(18):1963–1978. doi: 10.15252/embj.201593725

Figure 4. PBX1 improves the differentiation of neural progenitor cells toward mDA fate.

Figure 4

  1. Scheme of the differentiation protocol for hNES cells. The AF22 cells (NES cells derived from induced pluripotent stem cells) were infected at day 2 with TET‐on PBX1 lentiviral particles, and doxycycline (DOX) treatment started the day after.
  2. Representative immunofluorescence/confocal images showing that PBX1 overexpression increases the number of TH+ neurons after 8 days of differentiation of hNES cells (AF22 line derived from induced pluripotent stem cells).
  3. While the number of cells (Dapi+) in both conditions did not change, the number of TH+ cells increased 2.3‐fold in PBX1‐overexpressing cells.
  4. TH+ cells derived from the PBX1‐overexpressing hNES cells were also positive for mDAn markers such as LMX1A and PITX3.
Data information: All scale bars, 40 μm. Bars represent mean ± standard deviation. Biological replicates per condition and P‐value (t‐test) are indicated in the graphs.