Table 1.

Sequence of solvents used in the method of separation of total lipids and beta-carotene

Spotting of the sample	0.5 mL of hexane, 1.6% of diethyl-ether
Fraction A	One column volume of hexane, 1.6% of diethyl-ether
Fraction B	One column volume of hexane, 1.6% of diethyl-ether
Fraction C	One column volume of hexane, 1.6% of diethyl-ether
Fraction D	Five column volume of chloroform/methanol (1:1) Three column volume of methanol

Each fraction was then analyzed by thin-layer chromatography to validate the method resolution