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. 2017 Jan 31;18:117. doi: 10.1186/s12864-017-3506-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Overview of the ssRNA-seq transcriptome assembly and lncRNA identification pipeline. a Overview of the ssRNA-seq-based transcript reconstruction pipeline that was used to identify expressed transcripts in sequencing libraries. b The integrative pipeline for the stringent identification of lncRNAs in zebra finch dorsal skins. CPC: coding potential calculator; PLEK: predictor of long non-coding RNAs and messenger RNAs based on an improved k-mer scheme; HMMER (HMMER-3): Profiling protein sequence data using hidden Markov models