Table 1.
Mass spectrometry based method principles reported for clinical chemistry analytes in the Royal College of Pathologists of Australasia (RCPA) Quality Assurance Programs (QAP)
| Measurands included in the RCPAQAP Chemical Pathology Programs | Matrix | Program | Percentage of participants using MS method principle | Method principle |
|---|---|---|---|---|
| 3-methoxytyramine | Plasma | Plasma Metanephrines | 100% | LC-MS/MS |
| Urine | Urine Biogenic Amines | 58% | ||
| 4-hydroxy-3-methoxymethamphetamine (HMMA) / Vanillylmandelic Acid (VMA) | Urine | Urine Biogenic Amines | 25% | LC-MS/MS |
| 5-hydroxyindoleacetc Acid | Urine | Urine Biogenic Amines | 27% | LC-MS/MS |
| 17-hydroxy progesterone | Serum/Plasma | Endocrine | 45% | LC-MS/MS |
| 25-hydroxy vitamin D3 | Serum/Plasma | Endocrine | 10% | LC-MS/MS |
| Adrenaline | Urine | Urine Biogenic Amines | 23% | LC-MS/MS |
| Aldosterone | Serum/Plasma | Endocrine | 11% | LC-MS/MS |
| Aluminium | Serum | Trace Elements | 62% | ICP-MS |
| Urine | 83% | |||
| Amiodarone | Serum/Plasma | Special Therapeutic Drugs & Antibiotics | 25% | LC-MS/MS |
| Androstenedione | Serum/Plasma | Endocrine | 44% | LC-MS/MS |
| Arsenic | Urine | Trace Elements | 90% | ICP-MS |
| Whole blood | 88% | |||
| Benzodiazapines e.g. Oxazepam | Urine | Urine Toxicology | 30% | GC-MS (11%), LC-MS/MS (14%), LC-TOF/MS (5%) |
| Cadmium | Urine | Trace Elements | 83% | ICP-MS |
| Whole blood | 83% | |||
| Chromium | Serum | Trace Elements | 78% | ICP-MS |
| Urine | 80% | |||
| Clozapine | Serum/Plasma | Special Therapeutic Drugs & Antibiotics | 23% | LC-MS/MS |
| Cobalt | Serum | Trace Elements | 100% | ICP-MS |
| Urine | 90% | |||
| Copper | Serum | Trace Elements | 39% | ICP-MS |
| Urine | 60% | |||
| Cortisol | Saliva | Salivary Cortisol | 19% | LC-MS/MS |
| Serum/Plasma | Endocrine | 2% | ||
| Cyclosporin | Serum/Plasma/whole blood | Special Therapeutic Drugs & Antibiotics | 13% | LC-MS/MS |
| DHEAS | Serum/Plasma | Endocrine | 5% | LC-MS/MS |
| Dihydrotestosterone | Serum/Plasma | Endocrine | 63% | GC-MS (13%), LC-MS/MS (50%) |
| Dopamine | Urine | Urine Biogenic Amines | 24% | LC-MS/MS |
| Homocysteine | Serum/Plasma | Endocrine | 2% | LC-MS/MS |
| Homovanillic acid (HVA) | Urine | Urine Biogenic Amines | 23% | LC-MS/MS |
| IGF-1 | Serum/Plasma | IGF-1 / C-peptide | 3% | LC-TOF/MS |
| Iodine | Urine | Trace Elements | 89% | ICP-MS |
| Lead | Urine | Trace Elements | 77% | ICP-MS |
| Whole blood | 48% | |||
| Manganese | Serum | Trace Elements | 100% | ICP-MS |
| Urine | 88% | |||
| Whole blood | 78% | |||
| Mercury | Urine | Trace Elements | 100% | ICP-MS |
| Whole blood | 100% | |||
| Metanephrine | Urine | Urine Biogenic Amines | 48% | LC-MS/MS |
| Mycophenolate | Serum/Plasma | Special Therapeutic Drugs & Antibiotics | 33% | LC-MS/MS |
| Nickle | Urine | Trace Elements | 89% | ICP-MS |
| Noradrenaline | Urine | Urine Biogenic Amines | 21% | LC-MS/MS |
| Normetanephrine | Urine | Urine Biogenic Amines | 48% | LC-MS/MS |
| Oestradiol | Serum/Plasma | Endocrine | 1% | LC-MS/MS |
| Plasma free metanephrine | Plasma | Plasma Metanephrines | 93% | LC-MS/MS |
| Plasma free normetanephrine | Plasma | Plasma Metanephrines | 93% | LC-MS/MS |
| Progesterone | Serum/Plasma | Endocrine | 1% | LC-MS/MS |
| Selenium | Serum | Trace Elements | 82% | ICP-MS |
| Urine | 100% | |||
| Whole blood | 83% | |||
| Serotonin | Urine | Urine Biogenic Amines | 50% | LC-MS/MS |
| Sirolimus | Serum/Plasma/whole blood | Special Therapeutic Drugs & Antibiotics | 38% | LC-MS/MS |
| Sweat Chloride | Sweat | Sweat Electrolytes | 24% | ICP-MS |
| Tacrolimus | Serum/Plasma/whole blood | Special Therapeutic Drugs & Antibiotics | 17% | LC-MS/MS |
| Testosterone | Serum/Plasma | Endocrine | 9% | LC-MS/MS |
| Thallium | Urine | Trace Elements | 100% | ICP-MS |
| Tricyclic antidepressant general screen | Serum/Plasma | Special Therapeutic Drugs & Antibiotics | 13% | LC-TOF/MS |
| Vanadium | Urine | Trace Elements | 67% | ICP-MS |
| Vitamin A (retinol) | Serum/Plasma | Vitamins | 3% | LC-MS/MS |
| Vitamin B1 (thiamine pyrophosphate) | Whole blood | Vitamins | 4% | LC-MS/MS |
| Vitamin B6 | Serum/Plasma | Vitamins | 17% | LC-MS/MS |
| Zinc | Serum | Trace Elements | 39% | ICP-MS |
| Urine | 80% | |||
| Whole blood | 67% |
The percentage of mass spectrometric methods reported is based on the latest end of cycle or interim reports available on the RCPAQAP website. This data is presented with permission from the RCPAQAP Chemical Pathology Programs