Fig. 2.

Effects of Wy 14643 on 2-ClHA catabolism in primary mouse hepatocytes. Mouse hepatocytes were prepared from either C57BL/6J or PPAR-α^−/− mice, as indicated. Hepatocytes were pretreated with either Wy 14643 (10 μM), GW6471 (10 μM), or vehicle overnight and subsequently incubated with either 10 μM (A) or 50 μM (B) 2-ClHA for 24 h. The 2-ClAdA in the medium was analyzed as described in the Materials and Methods. Values represent mean ± SEM for n = 3. C: Primary hepatocytes from C57BL/6J mice were pretreated with or without Wy14643 (10 μM) overnight and then incubated with 50 μM of 2-ClHA for 24 h. Hepatocytes were collected for intracellular 2-ClHA analysis as described in the Materials and Methods. Values represent mean ± SEM for n = 3. **P < 0.01 for comparisons to control in (A) and (C). **P < 0.01 and ^##P < 0.01 as indicated in (B).