Fig. 5.

PPAR ligand analysis for 2-ClHA. In (A), (B), and (C), HEK293 cells were transfected with a luciferase reporter gene system to evaluate PPAR-α, PPAR-δ, and PPAR-γ activities, respectively. Cells were then treated for 24 h with the indicated concentrations of either HA, 2-ClHA, or isozyme-specific ligand (1 μM Wy 14643, GW0742, or rosiglitazone for PPAR-α, PPAR-δ, and PPAR-γ, respectively; all in closed circles). Values represent the mean ± SEM with n = 3. In some cases the error bars are within the symbol for the mean.