Fig. 2.

Degradation of nonoxidized two long-chain PC by LPLA2. The reaction mixture contained 49 mM sodium citrate (pH 4.5), 10 μg/ml BSA, liposomes (130 μM PL), and 40 ng/ml of recombinant mouse LPLA2 in 500 μl of total volume. The liposomes consisted of DODPC/DOPC/NAS (2.4:1:1.05, molar ratio). The reaction was initiated by the addition of recombinant LPLA2 and kept for 30 to 120 min at 37°C. The reaction products were extracted, separated with an HPTLC plate using a solvent system consisting of chloroform/acetic acid (9:1, v/v) (A), and plotted against the incubation time (B) as described in the Materials and Methods.