Skip to main content
. 2017 Jan 31;58(2):339–349. doi: 10.1194/jlr.M070730

Fig. 5.

Fig. 5.

Preferential hydrolysis of truncated ox-PCs by LPLA2. The reaction mixture contained 49 mM sodium citrate (pH 4.5), 10 μg/ml BSA, liposomes (130 μM PL), and 40 ng/ml of recombinant mouse LPLA2 in 500 μl of total volume. The liposomes consisted of DODPC/DOPC/truncated ox-PC/NAS (2.4:1:1:1.05, molar ratio). The reaction was initiated by the addition of recombinant LPLA2 and kept for 30 to 120 min at 37°C. The reaction products were extracted and separated with an argentate HPTLC plate using a solvent system consisting of chloroform/acetic acid (9:1, v/v) (upper panels). The reaction products, long-chain fatty acid, and 1-O-acyl-NAS in each reaction were quantified as described in Fig. 4 and were plotted against the incubation time (lower panels). PA, palmitic acid; OA, oleic acid; 1-O-Pal-NAS, 1-O-palmitoyl-NAS; 1-O-Ole-NAS, 1-O-oleoyl-NAS. The truncated ox-PC tested was POVPC, PGPC, PONPC, and PAzPC in A, B, C, and D, respectively.