Fig. 2.
Metabolism and distribution of NO2-OA in lipid fractions of NO2-OA-supplemented adipocytes. Fractionated lipid classes from adipocytes were analyzed by HPLC-MS/MS in negative ion mode after acid hydrolysis to determine the cellular content (picomoles per milligram protein) of NO2-OA (A), NO2-SA (B), dinor NO2-SA (C), tetranor NO2-SA (D), and hexanor NO2-SA (E). FFA fraction was not hydrolyzed. Two independent experiments were performed. Range (minimum-maximum) and mean of one representative experiment (n = 4) are shown.