Fig. 3.

Interaction of naturally-occurring FAs and fatty acyl-CoA with LXRα. Direct binding assay based on quenching of LXRα aromatic amino acid fluorescence emission when titrated with the following ligands: octanoic acid (A), decanoic acid (B), lauric acid (C), octanoyl-CoA (D), decanoyl-CoA (E), lauroyl-CoA (F), palmitic acid (G), palmitoyl-CoA (H), and T-0901317 (I). Data are presented as the change in fluorescence intensity (F₀-F_i) plotted as a function of ligand concentration. All values represent mean ± SE, n ≥ 3.