Fig. 6.

MCFA, lauric acid, or its metabolite, lauroyl-CoA, alter LXRα transactivation. COS-7 cells transfected with pSG5 empty vector, LXRα, PPARα, or both PPARα and LXRα were analyzed for transactivation of the SREBP-1c-LXRE-luciferase reporter construct in the presence of vehicle or 10 μM ligands. The y axis represents values for firefly luciferase activity that have been normalized to Renilla luciferase (internal control), where no ligand empty vector (pSG5) sample was arbitrarily set to 1. The bar graph represents the mean values (n ≥ 3) ± SE. *P < 0.05, **P < 0.01, and ***P < 0.0001. Asterisks denote significant differences due to ligand as compared with no-ligand controls. 25-HC, 25-hydroxycholesterol.