Fig. 7.

Decreased core accumulation on hepatic LDs of HCVcore^Tg15 mice with ASO-mediated knockdown of PLIN3. LD isolation from livers of male WT and HCVcore^Tg15 mice, 6 weeks of age, treated with either control (Con) or PLIN3 ASO for 6 weeks while being maintained on a MFD (n = 3 per group). A: Western blot analysis was performed on fractions. B–E: Western blot analysis was performed on the liver LD fraction of individual animals and quantitative analysis was performed relative to the WT control group on expression of core (C), PLIN3 (D), and CGI-58 (E). F–J: Lipids were extracted from LD fractions and lipid content was determined enzymatically for TGs (F), phosphatidylcholine (PC) (G), total cholesterol (TC) (H), free cholesterol (FC) (I), and esterified cholesterol (EC) (J). Data shown represent mean ± SEM. Levels not connected by the same letter are significantly different.