Table 1.
Gene | Primer sequence | |
---|---|---|
TGF-b1 | Sense | 5′-CACCATCCATGACATGAACC-3′ |
Antisense | 5′-TCATGTTGGACAACTGCTCC-3′ | |
Smad3 | Sense | 5′-GCTTGGTGAAGAAGCTCAAGAA-3′ |
Antisense | 5′-GCGTCCATGCTGTGGTTCAT-3′ | |
b-actin | Sense | 5′-CCTGCCTCTACTGGCGCTGC-3′ |
Antisense | 5′-GCAGTGGGGACACGGAAGGC-3′ |
RT-PCR – real time-polymerase chain reaction; TGF-β1 – transforming growth factor-β1; Smad3 – drosophila mothers against decapentaplegic protein 3.