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. 2016 May 11;24(1):55–64. doi: 10.3727/096504016X14586627440192

Figure 5.

Figure 5

2-Deoxy-d-glucose (2DG) alters EGFR expression. (A) FaDu cells were treated with 2DG with or without erlotinib (ERL) and analyzed for phosphorylated EGFR (pEGFR) and Akt (pAkt) expression and total EGFR (tEGFR) and Akt (tAkt) expression by Western blot. (B) FaDu, Cal-27, and SQ20B cells were treated with 2DG with or without ERL and analyzed for total EGFR (EGFR) expression. (C) FaDu, Cal-27, and SQ20B cells were pretreated with mannose (MAN) for 2 h before treatment with 2DG + ERL then analyzed for EGFR expression. Cells were treated with saline as a negative control (CON) and tunicamycin (TUN) as a positive control. β-Actin was used as a loading control.