Table 3.

Sgce expression in 3-month-old gene-trap mice using relative quantitative reverse transcriptase PCR (QRT-PCR). Primers 1 (85F/85R), all isoforms. Primers 2 (98F/98R), long isoforms. Primers 3 (E9F/E11bR), brain-specific isoform. Primers 4 (E9F/β-gal_R2), Sgce/lacZ fusion transcript. Values are referenced to wild-type (Sgce^+/+) cerebral cortex and presented as means ± standard error of the mean (SEM) (n = 8 mice/genotype).

Tissue	Sgce+/+				Sgcem+/pGt				SgcemGt/pGt
	Primers 1	Primers 2	Primers 3	Primers 4	Primers 1	Primers 2	Primers 3	Primers 4	Primers 1	Primers 2	Primers 3	Primers 4
Cerebral cortex	1.00 ± 0.02	0.70 ± 0.05	0.09 ± 0.01	0.00 ± 0.00	0.33 ± 0.04	0.00 ± 0.00	0.00 ± 0.00	0.15 ± 0.04	0.32 ± 0.03	0.00 ± 0.00	0.00 ± 0.00	0.18 ± 0.03
Cerebellum	2.70 ± 0.09	1.38 ± 0.08	0.17 ± 0.03	0.00 ± 0.00	1.09 ± 0.08	0.00 ± 0.00	0.00 ± 0.00	0.55 ± 0.03	0.99 ± 0.08	0.00 ± 0.00	0.00 ± 0.00	0.53 ± 0.04
Hippocampus	0.92 ± 0.02	0.62 ± 0.05	0.07 ± 0.01	0.00 ± 0.00	0.27 ± 0.04	0.00 ± 0.00	0.00 ± 0.00	0.15 ± 0.03	0.24 ± 0.02	0.00 ± 0.00	0.00 ± 0.00	0.14 ± 0.03
Midbrain	1.77 ± 0.05	1.27 ± 0.06	0.12 ± 0.01	0.00 ± 0.00	0.80 ± 0.09	0.00 ± 0.00	0.00 ± 0.00	0.38 ± 0.05	0.71 ± 0.06	0.00 ± 0.00	0.00 ± 0.00	0.30 ± 0.04
Striatum	1.27 ± 0.02	0.86 ± 0.05	0.11 ± 0.02	0.00 ± 0.00	0.46 ± 0.04	0.00 ± 0.00	0.00 ± 0.00	0.24 ± 0.03	0.39 ± 0.03	0.00 ± 0.00	0.00 ± 0.00	0.21 ± 0.03
Thalamus	1.47 ± 0.04	1.05 ± 0.04	0.10 ± 0.01	0.00 ± 0.00	0.60 ± 0.07	0.00 ± 0.00	0.00 ± 0.00	0.29 ± 0.03	0.57 ± 0.04	0.00 ± 0.00	0.00 ± 0.00	0.27 ± 0.04
Spinal cord	1.38 ± 0.02	1.11 ± 0.07	0.07 ± 0.00	0.00 ± 0.00	0.59 ± 0.05	0.00 ± 0.00	0.00 ± 0.00	0.25 ± 0.03	0.53 ± 0.03	0.00 ± 0.00	0.00 ± 0.00	0.23 ± 0.03
Liver	0.39 ± 0.02	0.28 ± 0.03	0.00 ± 0.00	0.00 ± 0.00	0.01 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.01 ± 0.00	0.01 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.01 ± 0.00