Table 4.
Unique sarcoglycan peptides identified in wild-type and Sgcem+/pGt whole brain ε-SG immunoaffinity purifications (IAPs). For each IAP (wild-type versus Sgcem+/pGt tissue, esg3788 antibody versus esg2-1358 antibody), all unique tryptic sarcoglycan peptides detected via mass spectrometry are included in the table. The ε-SG2 brain-specific peptides detected in wild-type tissue are in bold. Abbreviations: IAP, immunoaffinity purification; esg3788, anti-ε-SG antibody 3788; esg2-1358, anti-ε-SG2 antibody 1358.
| Protein | esg3788 IAP | esg2-1358 IAP | ||
|---|---|---|---|---|
|
| ||||
| Wild-type | Sgcem+/pGt | Wild-type | Sgcem+/pGt | |
| ε-SG | TPYSDGVLYGSPTAENVGKPTIIEITAYNRR | None | ||
| QVSTYQEVVR | QVSTYQEVVR | QVSTYQEVVR | ||
| EVENPQNQLR | EVENPQNQLR | |||
| FEVNGIPEER | ||||
| KLTEAmSL | ||||
|
| ||||
| β-SG | RNENLVITGNNQPIVFQQGTTK | RNENLVITGNNQPIVFQQGTTK | RNENLVITGNNQPIVFQQGTTK | None |
| LPSSSSGDQSGSGDWVR | LPSSSSGDQSGSGDWVR | |||
| THNILFSTDYETHEFHLPSGVK | ||||
| TSITSDIGmQFFDPR | TSITSDIGmQFFDPR | TSITSDIGmQFFDPR | ||
| LcMcADGTLFK | LcMcADGTLFK | |||
| GNEGVFIMGK | GNEGVFIMGK | |||
|
| ||||
| δ-SG | GVEINAEAGNMEAIcR | GVEINAEAGNMEAIcR | None | |
| LEGDSEFLQPLYAK | LEGDSEFLQPLYAK | LEGDSEFLQPLYAK | ||
| LLFSADDSEVVVGAER | LLFSADDSEVVVGAER | LLFSADDSEVVVGAER | ||
| VLGAEGTVFPK | VLGAEGTVFPK | VLGAEGTVFPK | ||
| VFEVcVcANGR | VFEVcVcANGR | |||
| VLTQLVTGPK | VLTQLVTGPK | VLTQLVTGPK | ||
| SRPGNALYFK | ||||
| SLVMEAPK | ||||
|
| ||||
| ζ-SG | ELHLQSTEGEIFLNADSIR | ELHLQSTEGEIFLNADSIR | None | |
| VLFSADEDEITIGAEK | VLFSADEDEITIGAEK | |||
| LEGISEFLLPLYVK | LEGISEFLLPLYVK | LEGISEFLLPLYVK | ||
| LGNLPIGSFSSSTSSSNSR | LGNLPIGSFSSSTSSSNSR | |||
| QTVYELcVcPnGK | QTVYELcVcPnGK | |||
| GVQVSAAAGDFK | GVQVSAAAGDFK | GVQVSAAAGDFK | ||
| STDLDIQELK | ||||
| VTGTEGAVFGHSVETPHIR | ||||