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Elution profile after size exclusion chromatography (A) through Superdex-200 HR 10/30 column of native (untreated) recombinant pea 2-Cys Prx and after its treatment with 10 mM DTT (+DTT) and 10 mM DTT + 5 mM GSSG (+DTT+GSSG). Asterisks indicate the samples subsequently analyzed by MALDI TOF/TOF. Pattern of oligomerization (B) analyzed by native PAGE and Coomasie staining of pre-reduced protein treated with GSSG or DTT, the non-treated protein (-DTT, -GSSG) being used as control.
