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Peroxidase activities of recombinant 2-Cys Prx and Prx IIF after the treatment with 5 mM GSSG. Peroxidase activity was measured in previously DTT-reduced proteins (control) and in GSSG-treated proteins after incubation with H2O2 for 10 min at 37°C, using trichloroacetic acid to stop the reactions, as described in material and methods. H2O2 was then quantified using the eFOX method.
