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Proposed mechanism of glutathionylation of the decameric reduced form of 2-Cys Prx after treatment with 5 mM GSSG, 5 mM GSNO or 750 μM SNP. Glutathionylation of the 2-Cys Prx can be caused directly by GSSG or GSNO or indirectly by GSH after nitrosylation of the protein by SNP. The glutathionylation of the decameric 2-Cys Prx induces dimerization of the protein.
