Table 1.
Glutathionylation of purified recombinant pea 2-Cys Prx analyzed by mass spectrometry MALDI-TOF/TOF after treatment of the pre-reduced (DTT) protein (2CPSH) with 5 mM GSSG or GSNO for 30 min (See Supplementary Figure S2).
| Protein | Sample | Cysteine | Modification |
|---|---|---|---|
| 2-Cys-Prx | 2CPSH | C52 | NO |
| C174 | NO | ||
| 2CPGSSG Decamer | C52 | NO | |
| C174 | YES 3105.40 (Cys-SG) | ||
| 2CPGSSG Dimer | C52 | NO | |
| C174 | YES 3105.43 (Cys-SG) | ||
| 2CPGSNO Decamer | C52 | NO | |
| C174 | YES 3105.46 (Cys-SG) | ||
| 2CPGSNO Dimer | C52 | NO | |
| C174 | YES 3105.39 (Cys-SG) |
The incorporation of a SG group in the Cys52 or Cys174 in the decameric and dimeric forms of the protein eluted from the size exclusion chromatography through Superdex-200 HR 10/30 is pointed in bold.