Table 2.
Glutathionylation of purified recombinant pea Prx IIF analyzed by mass spectrometry MALDI-TOF/TOF after treating the pre-reduced (DTT) protein (IIFSH) with 5 mM GSSG or GSNO for 30 min.
| Protein | Sample | Cysteine | Modification |
|---|---|---|---|
| Prx IIF | IIFSH | C59 | NO |
| C84 | NO | ||
| IIFGSSG | C59 | YES 2002.94 (Cys-SG) 2131.06 (Cys-SG) | |
| C84 | YES 2669.20 (Cys-SG) 2868.33 (Cys-SG) | ||
| IIFGSNO | C59 | YES 2002.96 (Cys-SG) 2131.04 (Cys-SG) | |
| C84 | YES 2669.20 (Cys-SG) 2868.33 (Cys-SG) |
The incorporation of a SG group in the Cys59 or Cys84 in the dimeric form of the protein eluted from the size exclusion chromatography through Superdex-200 HR 10/30 is pointed in bold.