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. 2016 Oct 10;17(12):1753–1765. doi: 10.15252/embr.201642358

Figure EV1. Subcellular localization of Rab22a in BMDC and JAWS‐II DC.

Figure EV1

  1. Confocal microscopy analysis showing endogenous Rab22a (red) co‐localizing with endogenous Rab11a (green) at the recycling center in BMDCs at steady state. The nuclear marker DAPI (blue) and DIC images are shown on the left panel. Scale bars: 5 μm.
  2. JAWS‐II DC was incubated with 3‐μm magnetic beads for 15 min at 37°C and chased for 0 and 45 min. Immunoblotting of purified phagosomes was analyzed for Lamp1 and Rab22a. Ten micrograms of protein was loaded on each lane for purified phagosomes.
  3. JAWS‐II DC was incubated with 3‐μm LB for 15 min at 37°C and chased for 0, 45, or 165 min. Rab22a staining on isolated phagosomes was measured by FACS at the indicated time periods. Data are representative of three independent experiments.

Source data are available online for this figure.