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. 2016 Oct 10;17(12):1753–1765. doi: 10.15252/embr.201642358

Figure EV3. Rab22a silencing does not affect antigen uptake, phagosomal degradation, or BMDC maturation.

Figure EV3

  1. Scramble and Rab22a KD JAWS‐II DCs were incubated with soluble FITC‐OVA for 60 min. DCs were fixed and analyzed by FACS. Histograms represent the fluorescent intensities of two different concentrations of soluble OVA after internalization at 37°C or the highest concentration at 4°C. Data are representative of three independent experiments.
  2. Representative FACS profiles showing the OVA staining on isolated phagosomes of 15 min, 1 h, and 3 h post‐internalization of 3‐μm LB from Scramble and Rab22a KD JAWS‐II DCs. The PI condition represents 3‐h phagosomes treated with proteases inhibitors. Data are representative of three independent experiments.
  3. Scramble and Rab22a KD BMDCs ± LPS‐treated were labeled for CD11c, CD80, IAb, and CD86, and were analyzed by FACS. The gatings on CD11c‐positive cells (red) are depicted in the left panels. The MFI values are specified between parentheses in the FACS profiles. Data are representative of two independent experiments.