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. 2016 Jul 25;35(23):2536–2552. doi: 10.15252/embj.201593565

Figure 3. Wrb disruption in IHCs causes a progressive hearing impairment in mice.

Figure 3

  • A–A″
    Grand averages of auditory brainstem responses (ABRs) from Wrb fl/fl:Cre A (red traces, SEM pink) and Wrb +/+:Cre A (black traces, SEM gray) mice. ABRs were recorded for three separate age groups (A–A″, as indicated in the graph) using click stimulation at 80 dB (peak equivalent) and 20 Hz stimulation rate. There was an age‐progressive reduction in ABR amplitude in Wrb fl/fl:Cre A mice.
  • B–B″
    ABRs from Wrb fl/fl:Cre A mice showed a progressive threshold increase, and Wrb +/+:Cre A mice and Wrb fl/fl mice lacking Cre‐recombinase had normal thresholds. Statistical comparison of the threshold at 12 kHz was done by Kruskal–Wallis test, and P‐values are from post hoc Dunn's multiple comparison test. Measurements in which no ABR was observed at the maximal available tone burst level (90 dB) or click level (120 dB) scored as 100 dB threshold. Data are represented as means ± SEM.