Figure EV4. Wrb‐deficient IHCs show normal hair bundle development, maintenance, and MET function (related to Figs 3 and 7).

• A
  Scanning electron micrographs of P6 and P10 IHCs of Wrb ^+/fl:Cre ^B (top) and Wrb ^fl/fl:Cre ^B (bottom) mice. No obvious deficit in bundle development was detected in Wrb‐deficient mice. Scale bars: 1 μm.
• B
  FM1‐43 uptake by P7 auditory (IHC and OHC) and vestibular hair cells shows no obvious difference in fluorescence levels between Wrb ^+/fl:Cre ^B (top) and Wrb ^fl/fl:Cre ^B (bottom) mice. Scale bars: 10 μm.
• C–F
  Representative MET responses to a family of stepwise deflections of the hair cell bundle for (C) IHCs and (E) OHCs in Wrb ^+/fl:Cre ^B (left) and Wrb ^fl/fl:Cre ^B (right) mice. Means ± SEM of the MET currents are presented in (D) for IHCs and (F) for OHCs, with individual values for each cell shown as circles. No statistically significant differences were found in peak MET current amplitude in Wrb‐deficient IHCs or OHCs.