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. 2016 Oct 26;35(23):2584–2601. doi: 10.15252/embj.201593132

Figure 1. MMS22L foci form during S phase of the cell cycle and colocalize with HR proteins.

Figure 1

  1. Immunofluorescence images of MMS22L foci formation at etoposide (ETP)‐induced DSBs during S, M, and G1 phases of the cell cycle in HeLa cells. Scale bar: 5 μm.
  2. Quantification of a representative experiment from (A) shows number of MMS22L foci per nucleus during indicated cell cycle phases (n = 2; n nuclei ≥ 60).
  3. Immunofluorescence images of CPT‐induced MMS22L, RPA2, BRCA2, and RAD51 foci in U2OS cells. Scale bar: 5 μm.
  4. Quantification of a representative experiment (n = 3) from (C) shows percentage of γH2AX, RPA2, BRCA2, and RAD51 foci colocalizing with MMS22L foci (n nuclei = 30; nuclei with ≥ 25 MMS22L foci were analyzed).
Data information: Boxplots in (B and D) represent distributions per nucleus; boxes indicate the 25–75 percentile and whiskers the 10–90 percentile. Horizontal lines mark the medians. Statistical analysis: Mann–Whitney U‐test; ***P ≤ 0.0001; ns, not significant.