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. 2016 Oct 26;35(23):2584–2601. doi: 10.15252/embj.201593132

Figure 3. MMS22L–TONSL localizes to active and stalled replication forks.

Figure 3

iPOND assays in Hela cells. Experiment schematics, resulting immunoblots, and quantifications are shown. Antibodies used for immunoblots are indicated throughout.
  • A
    Abundance of MMS22L–TONSL at active and HU‐stalled replication forks, and fully replicated DNA.
  • B, C
    MMS22L dynamics at stalled replication forks. Quantification (C) shows average relative abundance of indicated proteins detected in immunoblots (n = 3 except at time point 5 h, n = 1; error bars, SEM).
  • D, E
    Effect of MMS22L depletion on RAD51 levels at stalled replication forks. Quantification (E) shows average relative RAD51 abundance (n = 2; error bars, SEM).