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. 2016 Oct 26;35(23):2584–2601. doi: 10.15252/embj.201593132

Figure EV5. MMS22L forms foci upon replication fork collapse.

Figure EV5

  • A–C
    Representative immunofluorescence images (A) and boxplots of endogenous MMS22L (B) and RPA2 (C) foci distribution in U2OS cells treated with the indicated replication stress‐inducing agents. Cells in silico enriched in S phase based on increased nuclear RPA2 signal were analyzed. Quantification of a representative experiment is shown; n = 3. Scale bar: 5 μm.
  • D, E
    Representative immunofluorescence images of MMS22L and RPA foci in U2OS cells upon collapse of HU‐stalled replication forks by treatment with the ATR inhibitor (ATRi). Cells in silico enriched in S phase based on increased nuclear RPA2 signal were analyzed. Scale bar: 5 μm. Statistical analysis: Mann–Whitney U‐test; ***P ≤ 0.0001. n = 2.
  • F
    Western blot analysis of cell extracts from cells used in the experiments described in Fig 4A and B. Tubulin serves as a loading control.
  • G
    Western blot analysis of cell extracts from cells used in a representative EM experiment described in Fig 4C–F. GAPDH serves as a loading control.
Data information: The boxplots in (B, C, and E) represent distribution of foci numbers per nucleus; boxes indicate the 25–75 percentile and whiskers the 10–90 percentile. Horizontal lines mark the medians.