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. 2016 Oct 26;35(23):2584–2601. doi: 10.15252/embj.201593132

Figure 6. MMS22L–TONSL promotes DNA strand exchange by reducing RAD51 binding to dsDNA.

Figure 6

  • A
    Schematic representation of the DNA strand exchange assay with ssDNA.
  • B, C
    Strand exchange assay with ssDNA and RAD51, and with (C) or without (B) the addition of MMS22L–TONSL (75 nM).
  • D
    Quantification of (B, C) shows averages, n = 2; error bars, SEM.
  • E
    Averages of quantified strand exchange assays with ssDNA, RAD51 (270 nM), and varying concentrations of MMS22L–TONSL or MMS22L. n = 2; error bars, SEM.
  • F
    Quantification of electrophoretic mobility shift assays with dsDNA or ssDNA, RAD51, and MMS22L–TONSL or MMS22L. Averages shown, n = 2; error bars, SEM.
  • G
    A scheme of streptavidin pulldown assay performed in (H, I).
  • H
    A representative immunoblot showing the effect of increasing amounts of dsDNA on RAD51 binding to ssDNA (1 nM).
  • I
    A representative immunoblot showing effects of MMS22L–TONSL and MMS22L titration into reactions containing RAD51, ssDNA (1 nM), and dsDNA (10 nM), as indicated.