Figure EV1. Generation of CAMDI‐KO mice.
- Targeting strategy for CAMDI‐KO mice. The 3′ probes are used for Southern blot analysis. F1, F2, and R mark the primers used for genomic PCR.
- Genotypes of CAMDI mutant mice were determined by PCR on tail DNAs.
- Western blot analysis of E16 mouse brain lysate probed with anti‐CAMDI‐specific antibody to demonstrate an absence of CAMDI protein by CAMDI KO.
- Body weight analysis revealed normal body weight at birth and during juvenile age. n = 8 mice for each genotype.