Figure 5.

Sequential anti-Tim-3 treatment suppresses tumor growth and show better antitumor immune response. C57BL/6 mouse were injected 1 × 10⁶ MEER cells in the neck, after tumor progression (day 10) anti-mouse PD-1 mAb was given at five doses every 2 d, sequential anti-Tim-3 mAb treatment were given to one group of mice starting from day 22 for four doses every 2 d. Tumor volume were measured every 2 d, Mice TIL were harvested at day 31, and HPV E7-tetramer and PD-1 expression were assessed by flow cytometry. (A) Tumor growth curve up to day 31 was shown, (n = 5) significance was calculated by two-way ANOVA, *p < 0.05. (B) Representative flow plots and summary data showing %E7-tetramer⁺ cells in CD8⁺ T cells. (n = 5) Significance was calculated by ordinary one-way ANOVA, *p < 0.05.