Figure 4.

Deficiency of IL-21 results in an increase in theTh17 phenotype. (A) Comparative expression of RORγt and IL-17A in mesenteric lymph nodes from IL-21KO as compared with that from wild-type (WT) mice as determined by qRT-PCR (n = 5 per group) *p < 0.05. (B) Percent of naïve splenic CD4⁺ cells expressing IL-17A after polarization toward the Th17 phenotype as determined by flow cytometry with intracellular staining. (C) Representative density plots of IL-17A- and IFNγ-expressing cells after polarization are shown. Gating was performed on live, single cells.