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. 2017 Jan 31;12(1):e0170969. doi: 10.1371/journal.pone.0170969

Fig 2. Transgene basal expression and loss of expression by TATA box deletion.

Fig 2

Gene transfer assay results performed using the -0.1(ΔICR1-2)GFP (A) and the +20(ΔTATA)GFP (B) constructs. Structure and conventions are the same as in Fig 1. (a) Gastrula stage; (b) Pluteus stage. (A) Merged fluorescence and bright-field images (left) and GFP fluorescence images (right) from microinjected embryos are shown. (B) Triple-merged bright-field, GFP fluorescence and Texas Red fluorescence images (left) proving embryos are microinjected and GFP fluorescence images (right). × 20 magnification. Lv: lateral view; av: animal view.