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. 2017 Jan 17;13(1):e1006555. doi: 10.1371/journal.pgen.1006555

Fig 5. High fat diet alters relative Mtp expression levels in the cardiomyocytes and fat body.

Fig 5

(A, B) Relative mRNA levels of Mtp in larval fat body (A) and larval hearts (B) on NFD and HFD. Results are expressed as the fold difference compared with the NFD condition. Values were normalized with gapdh. ***p < 0.001, by two-tailed paired t-test analyzed over 3 independent experiments. (C) Relative mRNA levels of Mtp in in larval heart relative to larval fat body on HFD. Results are expressed as the fold difference. Values were normalized with gapdh. *p < 0.05, ***p < 0.001, by two-tailed paired t-test analyzed over 3 independent experiments. (D) Relative mRNA levels of Mtp in larval heart relative to larval fat body on NFD. Results are expressed as the fold difference. Values were normalized with gapdh. *p < 0.05, ***p < 0.001, by two-tailed paired t-test analyzed over 3 independent experiments. (E-E”) Representative confocal images of Mtp (E), Boca (E’), and Mtp and Boca (E”) stainings of the hearts in the Hand-Gal4 control third instar larvae on NFD. Scale bars represent 40 μm. Insets in E-E” represent the same portion of the heart tube (yellow boxes). Arrows in inset E mark Mtp and DAPI nuclear staining in three cardiomyocytes whereas arrows in inset E’ and inset E” mark Boca and DAPI staining and Mtp and Boca staining in the same three cardiomyocytes, respectively. (F-F”) Representative confocal images of Mtp (F), Boca (F’), and Mtp and Boca (F”) stainings of the hearts in the Hand-Gal4 control third instar larvae on HFD. Scale bars represent 40 μm. Insets in F-F” represent the same portion of the heart tube (yellow boxes). Arrows in inset F mark Mtp and DAPI nuclear staining in four cardiomyocytes whereas arrows in inset F’ and inset F” mark Boca and DAPI staining and Mtp and Boca staining in the same four cardiomyocytes, respectively. (G-G”) Representative confocal images of Mtp (G), Boca (G’), and Mtp and Boca (G”) stainings of the hearts in the Hand-Gal4−mediated mtp KD third instar larvae on HFD. Scale bars represent 40 μm. Insets in G-G” represent the same portion of the heart tube (yellow boxes). Arrows in inset C mark Mtp and DAPI nuclear staining in two cardiomyocytes whereas arrows in inset G’ and inset G” mark Boca and DAPI staining and Mtp and Boca staining in the same two cardiomyocytes, respectively.