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. 2017 Jan 17;13(1):e1006553. doi: 10.1371/journal.pgen.1006553

Fig 2. NUWA is mainly expressed in reproductive organs.

Fig 2

(A) The T-DNA insertion sites in nuwa/+. (B) Siliques of wild type (upper), nuwa/+ (middle) and complemented nuwa/- (lower). Red stars and the black arrow indicate early and late aborted seeds, respectively. Bar = 1 mm. (C) Nucleotide diversity in the genomic region centered on NUWA among 5 Brassicaceae species, i.e., A. thaliana, A. lyrata, C. rubella, B. rapa and E. salsugineum. (D) Real-time qPCR analysis of NUWA in various tissues. Error bars, mean ± SD. (E-M) GUS staining patterns in inflorescence (E), ovule before fertilization (F), ovule at 2–4 cell embryo stage (G), ovules at heart (H), torpedo (I), walking-stick (J) embryo stages, germinated seeds (K), 10-day-old seedlings (L) and rosette leaf (M) in pNUWA:NUWA-GFP-GUS transgenic plants. Arrowheads indicate lateral root primordia. Bars = 2 mm (E, L, M), 50 μm (F, G), 100 μm (H-J), 200 μm (M) or 10 μm (small figure in G).