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. 2017 Jan 17;13(1):e1006566. doi: 10.1371/journal.pgen.1006566

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© 2017 Kasparek et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — (A) Klk5^-/- line (depicted as Klk5^-/-Klk7^+/+Spink5^+/+), was used for preparation of Klk5^-/-Klk7^-/- mice (depicted as Klk5^-/-Klk7^-/-Spink5^+/+) by TALEN mutagenesis. Obtained Klk5^-/-Klk7^-/- mice were further crossed to a Flippase (FLPe) expressing mouse line to allow conditionally expressed Klk5, thus generating Klk7^-/- mice (depicted as Klk5^+/+(loxP)Klk7^-/-Spink5^+/+). Klk5^-/-, Klk7^-/- and Klk5^-/-Klk7^-/- lines were subsequently crossed with Spink5^+/- line to obtain Klk5^-/-Sp5^A135X/A135X, Klk7^-/-Sp5^A135X/A135X, and Klk5^-/-Klk7^-/-Sp5^A135X/A135X, i.e. double and triple KO lines. (B) Expression of Klk5, Klk7 and Spink5 at the mRNA level was quantified using qRT-PCR, n≥4 for each genotype, error bars represent standard deviations from mean.