(A) Expression of TSLP, TNFα, ICAM1, IL-1β and IL33 in the skin of E18.5 dpc wt, Sp5A135X/A135X, Klk5-/-Sp5A135X/A135X, Klk7-/-Sp5A135X/A135X, and Klk5-/-Klk7-/-Sp5A135X/A135X analysed by qRT-PCR, n≥5. Error bars represent standard deviations from mean; Data were analysed by One-way ANOVA followed by Bonferroni post-hoc tests. (B) Cytokine expression in the skin of P5 wt, Klk5-/-Sp5A135X/A135X, Klk5-/-Klk7-/-Sp5A135X/A135X and Klk5-/- analysed by qRT-PCR, n≥3. Error bars represent standard deviations from mean; Data were analysed by One-way ANOVA followed by Bonferroni post-hoc tests. (C) Analysis of serum levels of TNFα, IL-1β, IL-9 and IL-17 in 6 weeks old wt and Klk5-/-Klk7-/-Sp5A135X/A135X mice, n≥4. Error bars represent standard deviations from mean; Means were compared with a t-test, ns means “not significant“; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.