Assessment of organ inflammation. Data are presented as mean ± SEM from control and zymosan (zym) treated mice after intra-prostate injection of zym. Filled bars, prostate; unfilled bars, bladder. A: Myeloperoxidase activity (MPO) was not increased after intra-prostate injection of zymosan in either prostate or bladder tissue. B: CD4 protein, presented as fold vehicle treatment, was quantified from Western blots of prostate and bladder tissue taken from two vehicle and two zymosan-treated mice. C: Mast cell tryptase activity (units/mg tissue) increased significantly after intra-prostate injection of zymosan in both prostate and bladder. * p≤ 0.05 vs. vehicle, two-way ANOVA with Bonferroni posthoc test.