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. 2016 Dec 8;16(2):189–199. doi: 10.1080/15384101.2016.1261765

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© 2017 The Author(s). Published with license by Taylor & Francis

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — CASP8AP2 siRNA triggers activation of a p53 dependent S-phase checkpoint in normal but not in tumor cells. (A) Analysis of p53 target gene expression 3 d after knockdown of the indicated transcriptional regulators of histone genes. (B, C) Flow cytometry analysis of CASP8AP2 siRNA treated hTERT-RPE1 (B) and U2OS (C) cells indicates that non-replicated cells arrested in S-phase express a marker for DNA damage signaling (γ-H2AX; low right hand corner). (D, E) hTERT-RPE1 (D) but not U2OS (E) cells with activated DNA damage signaling have activated p53, based on expression of the p53 target-gene p21 (upper right hand corner).