Figure 2.

Exogenous SAMHD1 expression in HuT78 cells results in reduced colony formation. HuT78 vector control, and SAMHD1-expressing cells were plated in duplicate in 6-well plates at a density of 1 × 10³ cells/well in 1 ml methyl cellulose medium and were left in culture for 10 d. (A) Numbers of colonies were counted after 10 d following standard counting criteria (all colonies with >25 cells were counted). Data is presented as % of colony yield relative to vector control cells (left panel). **, p < 0.005, Representative images (20 × magnification) of colonies formed after 10 d (right panel). (B) Numbers of colonies of different sizes were counted after 10 d based on following standard counting criteria (small: <25 cells; medium: 25–50 cells, and large: >50 cells). Data is presented as percentages of colony yield relative to vector control cells. All the data presented are representative of 3 independent experiments.