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. Author manuscript; available in PMC: 2017 Jan 31.
Published in final edited form as: Biotechnol Bioeng. 2016 Sep 21;113(11):2403–2415. doi: 10.1002/bit.26017

Fig. 1. Genome-wide human siRNA library screen with HEK-CMV-luc2-Hygro cell line.

Fig. 1

(A) Workflow of the primary screen; (B) Distribution of siRNA effect on improved overall luciferase expression, The 119 siRNAs corresponding to 56 identified genes with strong enhancer MAD z-score (>3) are indicated as black circles. (C)Relative per cell luciferase yield as a function of the relative cell viability for each sRNA tested. The 20% increase cut-offs are highlighted and divide the entire population into quadrants (I, II, III and IV). siRNAs associated with top 56 genes with a MAD-z score>3 are indicated as red circles and those with MAD-z-score < 3 as orange circles.