Table 1.

Confirmed top 10 genes with 3 or more siRNAs yielding > 50% increase in luciferase activity. A 50% increase is biologically relevant and also corresponds to high statistical significance (>3 MAD-based z-scores).

Gene	Description	Overall luciferase yield (%)*,†	MAD-based z-score*	Viable cell density (%)*,†	MAD-based z-score*	Function
INTS1	Integrator Complex Subunit 1	172	4.55	96	0.46	3’- end processing of small nuclear RNAs U1 and U2
INTS2	Integrator Complex Subunit 2	165	4.17	89	−0.11	3’- end processing of small nuclear RNAs U1 and U2
HNRNPC	Heterogeneous Nuclear Ribonucleoprotein	163	4.10	97	0.48	Influencing pre-mRNA processing and other aspects of mRNA metabolism and transport
CASP8AP2	Caspase 8 Associated Protein 2	156	3.70	79	−0.86	Activation and regulation of CASP8 in FAS-mediated apoptosis
OAZ1	Ornithine Decarboxylase Antizyme	153	3.57	115	0.36	Inhibiting ornithine decarboxylase and inactivating the polyamine uptake transporter
PPP2R1A	Protein Phosphatase 2, Regulatory Subunit A, Alpha	153	3.56	96	0.46	Serving as a scaffold for Protein Phosphatase 2 assembly, essential for signal transduction pathways
PRPF19	Pre-mRNA Processing Factor 19	147	3.27	96	0.41	Spliceosome assembly and activating pre-mRNA splicing
CHAF1A	Chromatin Assembly Factor 1, Subunit A	138	2.80	82	−0.62	mediating chromatin assembly in DNA replication and DNA repair
CCT2	Chaperonin Containing TCP1, Subunit 2 (Beta)	126	2.23	85	−0.41	Chaperonin-mediated protein folding of actin, tubulin and other proteins
EEF1B2	Eukaryotic Translation Elongation Factor 1 Beta 2	124	2.13	85	−0.38	exchanging GDP bound to EF-1-α to GTP during the transfer of aminoacylated tRNAs to the ribosome

^*All values are medians of result from 6 siRNAs (3 siRNAs in primary screen and 3 siRNAs in validation screen) targeting a top gene.

^†Values are normalized to negative control siN.C. transfected cells (set as 100%).