Table 2.
The list of siRNAs targeting the polyamine pathway genes, OAZ1, OAZ2, OAZ3, ODC and AZIN1 and their effects on luciferase activity, cell viability and per cell luciferase yield. The data are from the primary siRNA screen, except for the last three additional siRNAs against OAZ1
| Gene Symbol | siRNA sequence | Luciferase activity (%) |
Viable cell number (%) |
Per cell luciferase yield (%) |
|---|---|---|---|---|
| OAZ1 | GCCUUGCUCCGAACCUUCAtt | 161.1 | 94.8 | 169.9 |
| GAUUAUCCUUGUACUUUGAtt | 144.5 | 101.9 | 141.8 | |
| GGCUGAAUGUAACAGAGGAtt | 127.6 | 94.9 | 134.5 | |
| CCGUAGACUCGCUCAUCUCtt | 174.4 | 85.4 | 204.2 | |
| GCUAACUUAUUCUACUCCGtt | 171.1 | 110.6 | 154.7 | |
| GGGAAUAGUCAGAGGGAUCtt | 92.8 | 102.7 | 90.4 | |
| OAZ2 | ACAUCGUCCACUUCCAGUAtt | 97.4 | 96.3 | 101.1 |
| GGACCUCCCUGUGAAUGAUtt | 95.4 | 86.0 | 110.9 | |
| CAGAUGGAUUAUUAGCUGAtt | 94.9 | 105.4 | 90.0 | |
| OAZ3 | CCGGGAAAGUUUGACUGCAtt | 101.5 | 75.8 | 133.9 |
| CCACGACCAGCUUAAAGAAtt | 90.5 | 95.76 | 94.5 | |
| GACUUUCACUUCCGCCUUAtt | 74.3 | 87.7 | 84.7 | |
| ODC1 | GAUGACUUUUGAUAGUGAAtt | 18.0 | 56.1 | 32.1 |
| GCAUGUAUCUGCUUGAUAUtt | 20.0 | 50.7 | 39.4 | |
| GCUUGCAGUUAAUAUCAUUtt | 28.4 | 60.8 | 46.7 | |
| AZIN1 | CACUCGCAGUUAAUAUCAUtt | 25.2 | 64.6 | 39.0 |
| CGAUGAACAUGUUAGACAUtt | 30.4 | 72.1 | 42.2 | |
| GCCCUCUGUUGGAUAUCUAtt | 45.6 | 72.1 | 63.2 |