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Total endothelial nitric oxide synthase (eNOS) protein expression in aorta normalized to β-actin (A), phosphorylated eNOS relative to total eNOS (B), and representative Western blots (C) are shown. Total eNOS protein expression was significantly greater in YC and OPTH compared with OC. There was a main effect of treatment for the ratio of phosphorylated eNOS/total eNOS. *Difference from OC, P < 0.05; n = 7 for each group.
